Culturing microorganisms

GCSE: Biology

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Background information
A microbe is any living thing that spends its life at a size visible sometimes only with a microscope. It is too tiny to be seen with the naked eye. Microbes are the oldest form of life on Earth. Some types have existed for billions of years. They may live as individuals or cluster together in communities. Microbes live in the water you drink, the food you eat, and the air you breathe. Right now, billions of microbes are swimming in your belly and mouth, and crawling on your skin, over 95% of microbes are good for you. Microbes include bacteria, viruses, fungi, algae, and protozoa. These single-cell organisms are invisible to the eye, but they can be seen with microscopes.
Yeast
The terms yeast and yeast like are vernacular terms for unicellular fungal organisms that reproduce by budding. This is an inadequate definition, mainly because
• some yeasts reproduce by fission
• many yeasts can produce mycelium or pseudohyphae under some nutritional and environmental conditions,
• many filamentous fungi may exist in a unicellular yeast-like form that reproduce by budding
The term "yeast" is of no taxonomic significance. It is useful only to describe a morphological form of a fungus. Most yeast have affinities to Ascomycota, but a small percentage have affinities to Basidiomycota
Materials
1. Microbial suspension
2. Agar
3. Yeast
4. Oven
5. Bunsen burner
6. Heat proof mat
7. Bench coat
8. Petri dish
9. Dampen disinfectant cloth
10. Microscope

Method

1. With a dampen disinfectant cloth the bench surface should be wipe down for more accurate experiment result.
2. Bunsen burner should be properly set up, if incorrectly set up it may cause fire.
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